Optichoc

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Optichoc

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Optichoc

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Web-Aspirate the media-Add 10ml PBS buffer to each flask. Wash the cells by carefully swirling the flask.-Aspirate the PBS buffer-Add 5ml of TrypLE to each flask and carefully swirl the flask.Incubate at 37C in a CO2 incubator for 10 minutes-Tap the side of the flasks to help dislodge the cells-Pool all resuspended cells in one T75 flask.-Transfer 1ml in one T75 …

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