Gfap-creert2
Web012849 B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J These GCE transgenic mice have a human glial fibrillary acidic protein (hGFAP) promoter sequence directing expression of a Cre … Web(D–F) GFAPCreERT2:DTA mice were i.p. injected with tamoxifen at days 1–5. (D) At days 5–8, mice exhibited a severe paralysis that rapidly pro- gressed to all four limbs and was accompanied by weight loss (mean ± SEM; n = 3–7/group; two-way ANOVA test).
Gfap-creert2
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WebApr 16, 2024 · To compare the transcriptomic profiles between normal and tumor tissues, we microdissected the ME region from three control and four Rax-CreERT2::Braf V600E mice at 2 months post tamoxifen ... WebJun 15, 2010 · Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic piglet. 07 November 2024. Eunhye Kim, Seon-Ung Hwang, … Sang-Hwan Hyun. ... For Tuj1 and GFAP double staining, cells were fixed with 4% paraformaldehyde, washed and incubated in primary …
WebMar 30, 2006 · GFAP-Cre transgenic mice have been developed to achieve gene recombination in astrocytes, the most abundant cells of the central nervous system, with pivotal roles during brain function and pathology. WebSep 7, 2024 · Here, Aldh1L1-CreER T2; GFP mice were injected with tamoxifen every 12 h (12 h) for five consecutive days and killed 28 days post-injection (dpi). (M,N) …
WebSep 1, 2011 · To avoid the contribution of GFAP + progenitors to gut neurogenesis during fetal/neonatal development and to directly test whether GFAP + cells give rise to neurons in the adult gut, we studied GFAP-CreERT2;Rosa-loxpEYFP mice. In these mice, Cre recombinase activity can be induced in GFAP-expressing cells by tamoxifen administration. WebNational Center for Biotechnology Information
WebTo inactivate Tsc1 gene expression in glial fibrillary acidic protein (GFAP)-positive cells (glia), we developed GFAP-Cre mice in which Cre-recombinase was expressed under the control of a human GFAP promoter. These GFAP-Cre mice were crossed with Tsc1flox/flox mice containing LoxP sites targeted to the Tsc1 gene ( Figure 1 (a) ).
WebGenetic confimation of hGFAP-CreERT2 mouse line and its tamoxifen inducible Cre expression. (A) Genetic map of hGFAP-CreERT2. (B) The primer sequences and PCR protocol of hGFAP-CreERT2 transgenic ... bob\u0027s water park floridaWebFeb 23, 2024 · Here, we determined whether microglial and astrocytic SRR contributes to synaptic deficits on CA1 apical dendrites after CCI injury in CX 3 CR 1 creErt2:SRR fl/fl and GFAP creErt2:SRR fl/fl mice, respectively (Figure S1). In SRR fl/fl mice, we observed a time-dependent reduction in spine density at 3 and 7 dpi as compared with sham mice … cllr lachlan bruceWebCentral hypothesis of thesis, approved August 2024: Astrocytes exhibit polarity by concentrating cytoplasmic glial fibrillary acidic protein (GFAP) to one pole of the soma and only in proximal ... cllr mark hawthorne mbeWebMar 8, 2024 · Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background Lech Kaczmarczyk, Nicole Reichenbach,... bob\\u0027s welding and equipmentWebOct 24, 2004 · The immunoreactivity of glial fibrillary acidic protein in mesangial cells and podocytes of the glomeruli of rat kidney in vivo and in culture. Biol. Cell. 90, 53–56 (1998). bob\u0027s welding anaheimWebI have a GFAP-CreERt2 mouse; hence want to verify transgenic expression exclusively in the brain but we are detecting endogenous Cre expression despite using Qiagen RNA extraction kit with DNase. cllr marion atkinsonWebMar 30, 2006 · GFAP-Cre transgenic mice have been developed to achieve gene recombination in astrocytes, the most abundant cells of the central nervous system, with … bob\u0027s welding anaheim ca